Kültürü Yapılan Bazı Mentha Türlerinin HPLC ile Fenolik Asitlerin Tayini ve Antioksidan Kapasitesi Belirlenmesi

Abstract

This study was designed to investigate the phenolic acid contents and antioxidant activities of 14 clones of mint species (M. spicata, M. piperita, M. villoso nervata and M. dumetorum) that cultivated in Turkey. The phenolic acid contents were analysed using HPLC. The antioxidant activities of these Mentha species were evaluated by free radical scavenging activity (DDPH•), cation radical scavenging activity (ABTS•+) and reducing power activity tests. Additionally, total phenolic content of mint species was determined spectrophoto metrically using Folin-Ciacelteau’s reagent. Phenolic acid contents of Mentha were evaluated in two subclasses: benzoic acid and cinnamic acid derivatives. The rosmarinic acid (which is cinnamic acid derivative) was the main phenolic acid for all tested plant material changing from 5482.6 to 31982.7 mg/kg dry weight concentrations. p-hydroxy benzoic acid (ranging from 30.5 to 236.4 mg/kg dry weight) and syringic acid (ranging from 95.4 to 375.2 mg/kg dry weight) were most abundant benzoic acids derivatives found in tested mint clones. According to DDPH● and ABTS●+ test results, while M. villoso nervata clone (originated from Osmaniye) has higher activity, M. spicata clone (originated from Manisa) has lower activity among the other clones. Reducing power activity of M. villoso nervata clone (originated from Osmaniye) was highest and M. spicata clone (originated from Konya) was lowest. It has been observed that the correlation between phenolic acid derivatives and antioxidant activity varied depending on used antioxidant activity methods. These results show that best Mentha for human health in terms of both phenolic acid content and antioxidant activity are the 3rd clone (M. spicata) and the 10th clone (M. villoso nervata) genotypes.