Abstract
Background Orbitally shaken flasks are commonly used at an early stage of bioprocess development with mammalian cells. In contrast to large-scale stirred-tank bioreactors, shaken flasks are usually operated in probe-independent bioprocesses, i.e. without strictly controlling the pH or dissolved oxygen concentration (DO). As a consequence, gas transfer issues are thought to limit the effectiveness of orbitally shaken flasks and bioreactors (OSRs). To define optimal operating conditions for probe-independent bioprocesses in OSRs, we tested the effects of the mass transfer coefficient of oxygen (kLa) on mammalian cell growth, recombinant protein production, and environmental conditions of the culture (pH, DO).
Highlights
Shaken flasks are commonly used at an early stage of bioprocess development with mammalian cells
As the working volume of the cultures increased in the 1-L orbitally shaken flasks and bioreactors (OSRs), the dissolved oxygen concentration (DO) decreased (Fig. 1b)
Our results indicate that the kLa is a good parameter to predict suitable conditions for cell cultures in probe-independent OSRs
Summary
Shaken flasks are commonly used at an early stage of bioprocess development with mammalian cells. In contrast to large-scale stirred-tank bioreactors, shaken flasks are usually operated in probe-independent bioprocesses, i.e. without strictly controlling the pH or dissolved oxygen concentration (DO). Gas transfer issues are thought to limit the effectiveness of orbitally shaken flasks and bioreactors (OSRs). To define optimal operating conditions for probe-independent bioprocesses in OSRs, we tested the effects of the mass transfer coefficient of oxygen (kLa) on mammalian cell growth, recombinant protein production, and environmental conditions of the culture (pH, DO)
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