Kitosan ve EDTA Solüsyonlarının Sonik ve Ultrasonik Sistemler ile Aktive Edilmesinin Dentin Mikrosertliğine Olan Etkisi

Abstract

Objective: The aim of this in vitro study is to assess the effect of activation of EDTA and chitosan by different irrigation activation systems on the microhardness of root canal dentin. Material and Methods: A total of 60 single-rooted extracted mandibular premolars were prepared and randomly distributed into two groups (n=30) based on the final irrigant: Group 1, 0.2% chitosan; Group 2, 17% EDTA. Then, specimens of both groups were randomly divided into three subgroups (n=10) based on the irrigant activation; Subgroup A (Sonic), Subgroup B (Ultrasonic) and Subgroup C (Conventional irrigation, control group). The specimens (n=60) were embedded into resin blocks and sectioned horizontally 1 mm thick sections from 2,5 and 8 mm levels from the apex. All samples were used to determine the microhardness of dentin using a Vicker's microhardness tester. The data were analyzed using the three-way analysis of variance (ANOVA) and Tukey post-hoc tests to detect the effects of the independent variables (final irrigation solution, final irrigation techniques, and root canal thirds) on microhardness. Results: The three-way ANOVA indicated that chitosan exhibited a significantly lower microhardness value than EDTA (p<0.001). Also, regardless of the usage of final irrigation, both PUI and EA had significantly lower microhardness than CI (p<0.001). Conclusion: Chitosan as compared to EDTA (i), sonic (EA) and ultrasonic (PUI) final irrigation activation methods as compared to the traditional syringe method (CIS) (ii), apical and mid regions of the root as compared to its coronal region (iii), showed lower microhardness values.