KIR-HLAC genotyping in married couples with early reproductive losses of unknown genesis

Abstract

Aim. KIR-HLAC genotyping in married couples with early idiopathic pregnancy loss. Methods. DNA extraction and purification, PCR-SSP, agarose gel electrophoresis. Results. The spectrum of KIR genes was analyzed and the frequency of KIR genotypes in women with early reproductive losses was established. The most common (77.78 %) was the AB genotype, 20.37 % had the AA genotype, and 1.85 % had the BB genotype. HLAC genotyping of couples with regular early reproductive losses showed the C1/C2 genotype of the HLAC gene in 40.74 % of women and 44.44 % of men. The frequency of C1/C1 genotype in women was 27.78% versus 38.89 % in men. The C2/C2 genotype of the HLAC gene was detected in 31.48 % of women and 12.96 % of men. According to the results of KIR-HLAC analysis of genotyping of married couples with early reproductive losses, a high/significant risk of reproductive losses of immunological genesis was found in 55.56 % of cases. Conclusions. KIR-HLAC genotyping is a genetic test that allows to assess the risks of the embryo being rejected by the maternal immune system, and thus to direct medical interventions in order to achieve a successful pregnancy.
 Keywords: early reproductive losses, KIR, HLAC.