Abstract
MILLER et al.1–3 presented evidence that kinetin when used together with indolyl-3-acetic acid increased cell proliferation in tobacco callus tissue. These authors isolated kinetin from the DNA of herring sperm and calf thymus. Since that time additional evidence indicating that kinetin acts as a mitotic stimulant on plant tissues has been presented. Guttman4 reported a larger proportion of division figures in the meristematic region of roots of Allium cepa following 4 and 24 hr. treatment with kinetin. Torrey5 noted that kinetin together with 2,4-D initiated frequent mitoses in tetraploid muclei in excised callus tissue from mature pea roots. In the absence of kinetin the tetraploid nuclei remained in the resting state. The action of kinetin on animal tissues is still controversial. Cudkowicz6 treated mice with kinetin following irradiation and compared these with mice that were simply irradiated. He noted that irradiation inhibited mitoses in the crypts of Lieberkuhn. The return to the normal mitotic rate was enhanced by treating the mice with kinetin following irradiation.
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