Abstract
Electroporation involves the application of an electric pulse that creates transient aqueous channels (electropores) across the lipid bilayer membranes. Here, we describe an instrument set up suitable to record ultraweak light emission from human erythroleukemia K562 cells during and immediately after delivery of electric pulses. Most of light was emitted in the first seconds after each pulse, following a complex decay which can be fitted by a double exponential equation characterized by two different time constants ( T 1 and T 2), both in the order of seconds. ( T 1 was approximately 10-fold shorter than T 2 and both time constants were dependent on field strength of the electric pulse. The effect of various antioxidants on the amount of emitted photons and on ( T 1 and T 2) values was investigated, in order to shed some light on the chemical species responsible for cellular luminescence.
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