Abstract
In the present work, the kinetics of Mn 2+ ion and radiation induced free radical in gamma irradiated soybean were investigated in detail using ESR spectroscopy in the temperature range of 130–400 K. Like other legumes, such as lentil, pea and broad bean, the EPR spectrum of untreated soybean is composed of an equally spaced sextet due to Mn 2+ ion and a singlet resonance line both appearing at g=2.00±0.0010. Radiation was observed to cause an exponential increase in the intensity of the singlet resonance line but not creating any effect on Mn 2+ ion resonance line intensities in the studied dose range of 1.25–15 kGy. Reversible increases of Mn 2+ and free radical signals were observed below room temperature. Above room temperature, they both decreased and these decreases were irreversible. The kinetic features of Mn 2+ and free radical signal intensities were studied by annealing the samples at predetermined temperature for predetermined time over the temperature range of 308–373 K. Ground soybean samples were used throughout the experiment to avoid any possible problems arising from the microwave cavity filling factor.
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