Abstract
In the light of the scheme presented by Walsh et al. (1978, Meth. Enzymol. 53, 437-448), Waley (1980, Biochem. J. 185, 771-773) and Tatsunami et al. (1981, Biochim. biophys. Acta, 662, 226-235) made a detailed study for the kinetics of suicide substrates. However, the effects of the enzyme reaction intermediate and product were not considered in their studies. Equations for the kinetics of suicide substrate were derived for a more reasonable scheme. A plotting method was proposed to determine the kinetic parameters and it has been shown that suicide substrate can be used to study the formative mechanism of enzyme-catalyzed reaction intermediate.
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