Kinetics of Reactive Oxygen Species and Total Antioxidant Capacity during Moderate Treadmill Running after 8 Weeks of Endurance Training

Abstract

The purpose of this study is to investigate the effect of aerobic exercise intensity on the kinetics of reactive oxygen species and total antioxidant capacity during the moderate intensity of treadmill running. Twelve healthy collegiate male students participated and were randomly divided into two groups. Each group of subjects trained at 80% and 50% HRmax respectively for 30 min per session, 3 times per week for 8 weeks. After 48 hours of the training session, treadmill running task was imposed on each group at 70% of VO2max for 90 minutes. Metabolic data were monitored using a gas analysis system. Blood samples were taken from the finger before exercise and after 30 minutes, 60 minutes, and 90 minutes of exercise for measuring the blood lactate, derivatives of Reactive Oxygen Metabolites (d‐ROM), and biological antioxidant potential (BAP). All data were analyzed by two‐way ANOVA of SPSS 18.0. The differences between pre and post training were significant in d‐ROM and BAP (p<.05). The changes of d‐ROM were significantly increased by aerobic training, and there were significant differences among exercise durations (p<.05). The changes of BAP were not significantly different between training intensity groups, and there were significant differences among exercise durations (p<.05). As shown above, the occurrence of d‐ROM and BAP were altered by training intensity and exercise duration. In other words, the occurrence of reactive oxygen by high intensive training was abundant and there were significant changes in 30 minutes of exercise based on exercise duration. On the other hand, there were significant changes in total antioxidant capacity in 60 minutes of exercise based on exercise duration. In conclusion, low‐intensity training and within 60 minutes of exercise might be suitable in the point of defending the cellular damage caused by exercise.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.