Kinetics of olive oil hydrolysis by Candida cylindracea lipase

Abstract

In this study, we investigated the kinetics of olive oil hydrolysis in an oil-in-water emulsion in the absence of emulsifier. As this reaction is catalyzed by the lipase acting at the interface of oil and aqueous phase, the heterogeneous character of the process was taken into account during the formulation of the model of enzymatic hydrolysis of olive oil. Five kinetic equations were derived from a general mechanism. Their parameters were optimized using a multiresponse method considering all experimental data obtained at different enzyme concentrations. A model that incorporated the distribution of enzyme between the aqueous phase and oil-water interface was chosen as the best one.