Kinetics of nitrate uptake by New Zealand marine macroalgae and evidence for two nitrate transporters in Ulva intestinalis L.

Abstract

Kinetic constants were determined for nitrate uptake in three species, Pterocladiella capillacea (S.G. Gmelin) Santelices et Hommersand (Rhodophyceae, Gelidiales), Ulva intestinalis L. (Chlorophyceae, Ulvales) and Xiphophora chondrophylla (Turner) Montagne ex Harvey (Phaeophyceae, Fucales), of New Zealand macroalgae, with Km values ranging from 10 to 17 μM and Vmax values from 3 to 65 μmole g−1 dry weight h−1. There was no effect of ammonium on nitrate uptake by Pterocladiella capillacea or Xiphophora chondrophylla. Ammonium inhibited nitrate uptake by 40% in Ulva intestinalis from a site with relatively low seawater ammonium concentrations. In contrast, U. intestinalis from an ammonium-enriched site had lower rates of nitrate uptake that were insensitive to inhibition by ammonium. It is suggested that there are (at least) two transport systems for nitrate in U. intestinalis; a constitutive transporter, which is insensitive to ammonium, and a transporter that is sensitive to ammonium inhibition and down-regulation by ammonium; the implications of this for our understanding of macroalgal blooms is discussed.