Kinetics of methyl viologen reduction by hydrogen catalyzed by hydrogenase from Desulfovibrio vulgaris

Abstract

The reduction of methyl viologen by hydrogen with hydrogenase was studied kinetically. The initial rate of the reduction was expressed as ▪ where k′, K 2, and K 3 are constants and [ S′] is the concentration of methyl viologen. According to this equation, a sequential mechanism was proposed. By combining the mechanism of hydrogen production from the reduced methyl viologen, a reaction mechanism for the reduction and oxidation of methyl viologen was proposed.