Abstract
1. 1. Rat mammary gland arginase is a metallo-enzyme dependent on Mn 2 +, which can be only partially substituted by Cd 2 +. 2. 2. Reconstitution of the activity of dialyzed arginase by manganese is a two-phase process; the second phase is independent of the cation concentration, with a half-time recovery ( t 1 2 ) of 10.77 min. 3. 3. The apparent K m for Mn 2+ is 280 μ M and 10.5 μ M for enzyme dialyzed for 24 and 72 hr, respectively. 4. 4. Treatment with 5 mM EDTA at pH 6 totally inhibits enzyme activity, which is reconstituted by Mn 2+. 5. 5. Results obtained with iodocetamide treatment suggest the existence of sulphydryl groups accessible only when the enzyme is dialyzed.
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