Abstract
Heart and white skeletal muscle of ocean pout (Macrozoarces americanus) express only a single lactate dehydrogenase isozyme based on electrophoretic and immunological analysis. The enzyme has been partially purified and its kinetic properties elucidated in both the pyruvate reductase and lactate oxidase directions. Km values and responses to changing pH catagorize the enzyme as a classical skeletal muscle type lactate dehydrogenase. The kinetic parameters are assessed with respect to known in situ carbon flux rates through this locus. It is concluded that the enzyme data per se provide little insight into either the dominant direction or the net maximal rate of carbon flow.
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