Abstract
The distribution of putative liver estrogen receptors (ER) between redissolved 30% ammonium sulfate-precipitated liver cytosol (AS cytosol) and dense sucrose-Triton-X-100- purified liver nuclei (purified nuclei) has been examined in female and male rats. The total and occupied ER of AS cytosol and purified nuclei were determined by exchange assays using 17β-[3H]estradiol. After administration of 100 μg nonradioactive ethinyl estradiol to either adult females or adult males, the total ER of AS cytosol decreased. Concomitant with the decrease in ER of AS cytosol, the total and occupied ER of purified nuclei increased. The binding specificity of the total ER of purified nuclei from ethinyl estradiol-treated males resembled that of the total ER of AS cytosol from vehicle-treated (control) males. In females, a maximal level of ER was observed in purified nuclei 0.5 h after ethinyl estradiol administration. The levels of ER were lower at 1, 2, and 4 h. Unlike the female, high levels of ER in the male were maint...
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
