Abstract
This study was done to extract hydrocarbon compounds from El-Lajjun oil shale using biosurfactant produced from two strains Rhodococcus erythropolis and Rhodococcus ruber. The results have shown that, optimal biosurfactant production was found using naphthalene and diesel as a carbon source for R. erthropolis and R. ruber, respectively. Optimum nitrogen concentration was 9 g/l and 7 g/l for R. erthropolis and R. ruber, respectively. Optimum K 2HPO 4 to KH 2PO 4 ratio, temperature, pH, and agitation speeds were 2:1, 37 °C, 7 and 200 rpm. Under optimal conditions R. erthropolis and R. ruber produced 5.67 and 6.9 g/l biosurfactant, respectively. Maximum recovery of oil achieved with hydrogen peroxide pre-treatment was 25% and 26% at biosurfactant concentration of 8 g/l and 4 g/l for R. erthropolis and R. ruber, respectively. The extent desorption of hydrocarbons from the pre-treated oil shale by biosurfactant were inversely related to the concentration of high molecular weight hydrocarbons, asphaltenes compounds. Pre- treatment of oil shale with hydrogen peroxide produced better improvement in aromatic compounds extraction in comparison with improvement which resulted from demineralization of the oil shale.
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