Abstract
A strain of Bacillus brevis has been isolated and identified based on biochemical results. Phenol biodegradation in a batch reactor was studied using the pure culture of B. brevis. The isolated strain was optimized for various environmental conditions and the biodegradation of phenol was maximum at pH 8.0, 5% (v/v) of inoculum size and without any co-substrate. The kinetics of biodegradation according to Haldane's equation μ = μ max S/( K s + S + ( S 2/ K i)) adequately describes cell growth with kinetic constants in the ranges μ max = 0.026–0.078 h −1, K s = 2.2–29.31 mg/l, K i = 868.0–2434.7 mg/l. These values are specific for this organism and we have compared with literature for pure or mixed cultures degrading phenol.
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