Abstract
A continuous bioprocess for the specialty chemical catechol has been developed usingBacillus stearothermophilus BR321. Themeta pathway for phenol degradation has been blocked at the catechol 2,3-dioxygenase enzyme using transposon mutagenesis. As a result, the organism grows on a dilute growth medium supplemented with phenol and accumulates catechol. In batch culture, catechol concentrations up to 1 mM were obtained. A kinetic model has been developed to describe batch growth for different initial phenol concentrations. In continuous culture conducted at a diluation rate of 0.12 hr−1, steady states were obtained for feed phenol concentrations up to 0.24 mM, resulting in catechol concentrations up to 0.08 mM. Above this phenol concentration, oscillatory dynamics were observed.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
