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Abstract
The kinetics of 32 P end-labeled DNA binding from solution to electrochemically prepared polypyrrole (PPy) thick films are investigated. These data were found to fit a simple diffusion-limited binding model with low phenomenological activation energy (5 kcal/mol), consistent with diffusion-limited binding kinetics. These data suggest that the DNA binds to PPy without significant alteration of its structure. The binding of double-helical DNA to PPy was found to be dependent upon the presence of the electrooxidized positively charged surface functional groups on PPy. DNA adsorption kinetics are only moderately affected by changes in ionic strength and pH in the 5-9 range. A twofold increase in binding rate is observed for single-stranded DNA compared to the double-stranded DNA binding level
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