Abstract
Experiments with environmentally relevant concentrations of Cu in glass aquaria revealed that Cu was quickly removed from water. Cubic regression of Cu concentration against time showed that maximum rate of removal was around 69.34-72.11h irrespective of treatment. The 96h LC50 value of Cu was respectively 0.18, 0.19 and 0.35mg/L for fish Cyprinus carpio, crustacean Diaptomus forbesi and worm Branchiura sowerbyi. Normalizing the lethal values and plotting them against time it was observed that there was sharp differences in mortality over time between the organisms and 96h lethal values could misrepresent susceptibility of the organisms to Cu. Treatment of 0.1mg/L of Cu in water resulted in accumulation of 10.57, 4.38, 1.46 and 2.44µg/g of Cu, respectively in sediment, worm, crustacean zooplankton and whole body of fish. But, Cu deposited in high concentrations in gut and liver of fish indicating that Cu was principally accumulated through food.
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