Abstract
1. 1. The kinetic parameters ( V max , K t ) of Na +-dependent d-glucose transport into brush border membrane vesicles (BBMV) from sheep and pig jejunum were determined. Due to the fermentation of ingested carbohydrates in the rumen the small intestine of ruminants (sheep) has to absorb much less glucose than the small intestine of monogastric omnivores (pigs) or herbivores. 2. 2. Kinetic analysis of the concentration dependence of d-glucose transport revealed a ten-fold smaller V max value combined with a five times lower K t value in sheep BBMV compared with pig BBMV. 3. 3. The V max value for l-leucine transport did not differ between the two species investigated, whereas the K t value in the sheep exceeded that in the pig. 4. 4. It is concluded from these results that the mechanism for Na +-dependent d-glucose transport in ruminants is adapted to the small amounts of carbohydrates reaching the small intestine.
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