Kinetics of cytokine production in human T-cell cultures

Abstract

RATIONALE: In in vitro culture systems cytokines are used as hallmarks of T-cell responses and to distinguish between Th1, Th2, and Treg responses. Recently, detection systems that detect several cytokines in one sample have been introduced. This calls for a reevaluation of the kinetics of T-cell cytokine production in culture systems to ensure that the all cytokines measured are detectable at the time of sample collection and that cytokine profiles measured reflects the complete response.