Abstract
RATIONALE: In in vitro culture systems cytokines are used as hallmarks of T-cell responses and to distinguish between Th1, Th2, and Treg responses. Recently, detection systems that detect several cytokines in one sample have been introduced. This calls for a reevaluation of the kinetics of T-cell cytokine production in culture systems to ensure that the all cytokines measured are detectable at the time of sample collection and that cytokine profiles measured reflects the complete response.
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