Abstract
The kinetics of CO rebinding with isolated Bacillus FTU caa 3-type oxidase and with solubilized Escherichia coli membranes (GO103 strain) containing the o-type oxidase as the main O 2reducing enzyme were studied under reducing conditions by laser flash photolysis of the CO-oxidase complexes. The spectra of the optical absorbance changes upon photolysis were characteristic of CO- caa 3 and CO- o-oxidase complexes in Bac. FTU and E.Coli, respectively. Small quantities of d-type oxidase in E.Coli GO103 membranes were detected. The kinetics of CO reassociation with reduced caa 3 and o-type oxidases were monophasic with τ 25–30 ms in both cases.
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