Abstract
Citrate uptake was studied in growing cells of Leuconostoc mesenteroides subsp. mesenteroides. A Michaelis-Menten pattern with the dianionic form of citrate as the limiting substrate has been proposed. It was validated for different fermentations varying the initial citrate concentrations and the pH medium. This latter did not modify the rate of the process which was clearly confirmed using experiments with resting cells. The model was used to compare the kinetics of citrate consumption between several strains of Leuconostoc mesenteroides subsp. mesenteroides, Leuconostoc mesenteroides subsp. cremoris and Leuconostoc lactis.
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