Abstract
The objective of this work was to investigate the effect of dietary supplementation with essential fatty acids on the kinetics of macrophage accumulation and giant cell formation in Nile tilapia (Oreochromis niloticus). The supplementation sources were soybean oil (SO, source of omega 6, n‑6) and linseed oil (LO, source of omega 3, n‑3), in the following proportions: 100% SO; 75% SO + 25% LO; 50% SO + 50% LO; 25% SO + 75% LO; and 100% LO (four replicates per treatment). After a feeding period of three months, growth performance was evaluated, and glass coverslips were implanted into the subcutaneous connective tissue of fish, being removed for examination at 2, 4, 6, and 8 days after implantation. Growth performance did not differ between treatments. Fish fed 100% linseed oil diet had the greatest macrophage accumulation and the fastest Langhans cell formation on the sixth day. On the eighth day, Langhans cells were predominant on the coverslips implanted in the fish feed 75 and 100% linseed oil. n‑3 fatty acids may contribute to macrophage recruitment and giant cell formation in fish chronic inflammatory response to foreign body.
Highlights
The participation of fatty acids in nonspecific defense responses is attributed to the production of eicosanoids with pro‐inflammatory activity (Balfry & Higgs, 2001)
Multinucleated giant cells (MGC) formation occurs as an attempt to involve the foreign body in order to isolate it
The objective of this work was to investigate the effect of dietary supplementation with essential fatty acids on the kinetics of macrophage accumulation and giant cell formation in Nile tilapia (Oreochromis niloticus)
Summary
The participation of fatty acids in nonspecific defense responses is attributed to the production of eicosanoids with pro‐inflammatory activity (Balfry & Higgs, 2001). Fish fed diets rich in n‐3 and n‐6 fatty acids present greater B lymphocyte response and survival after challenge with Aeromonas salmonicida and Vibrio anguillarum (Thompson et al, 1996). They have higher antibody titers when immunized against Edwardsiella ictaluri (Fracalossi et al, 1994). MGC formation occurs as an attempt to involve the foreign body in order to isolate it. These macrophage activities are regulated by chemical mediators and
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