Abstract
Deoxyribonucleic acid (DNA) synthesis in control and equine abortion virus (EAV)-infected L-M cell suspension cultures was examined by measuring the incorporation of labeled thymidine (TdR-2- 14C) and deoxycytidine (CdR-2- 14C) into the DNA fraction. Employment of methylated-albumin kieselguhr (MAK) column chromatography to separate viral and cellular DNA revealed the relative amounts being synthesized during infection. These studies showed that there was a marked and progressive inhibition in cellular DNA synthesis beginning as early as 2–4 hours post infection (PI) and continuing throughout the cycle. Viral DNA synthesis was initiated by 4–6 hour PI and the rate increased rapidly; by 12–14 hours (the period of maximal EAV DNA synthesis), it was approximately 15 times that observed for cellular DNA. The kinetics of biosynthesis of both species of DNA throughout the infection cycle are described and possible mechanisms responsible for inhibition of cellular DNA synthesis are discussed.
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