Abstract
The biodegradation of TCE was studied in a laboratory scale biofilter packed with wood charcoal and inoculated with mixed culture of methanotrophs isolated from local soil. The removal efficiency was found to be higher than 90% up to an inlet load of 5.1 g/m 3 h. The maximum elimination capacity was 6.7 g/m 3 h at an inlet loading rate of 11.3 g/m 3 h. The reaction constants EC max, K s and K i calculated from the experimental results are also presented. The biodegradation process is found to be inhibited at higher TCE concentration. The carbon dioxide production rate has been found to be a linear function of elimination capacity. The DNA finger printing techniques has indicated the presence of functionally active methanotrophic community including Methylocystis sp. in the biofilter.
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