Kinetic study on enzymatic esterification of tuna fish oil fatty acids with butanol

Abstract

Abstract Docosahexaenoic acid (DHA) is an important polyunsatured fatty acid (PUFA) which can be purified from tuna fish oil fatty acids by selective enzymatic esterification. The present paper investigates the kinetic study for selective esterification of tuna fish oil fatty acids with butanol catalyzed by Rhizopus oryzae lipase (ROL) in biphasic solvent system. Under the most suitable reaction conditions, 76.2% esterification was achieved in 24 h. Different kinetic models for esterification given by Segel [1] , Oliveira et al. [2] , Gogoi et al. [3] , and Kraai et al. [4] were tested for fitting the esterification data and the model given by Oliveira et al. [2] was found to be most suitable. The model given by Prazeres et al. [5] for hydrolysis was also tested for esterification and the model with second order product inhibition was found to provide better match between the predicted and experimental values than that of model by Oliveira et al. [2] . The kinetic model was fitted using MATLAB ® to determine the best kinetic parameters. The average value of kinetic constants using the model given by Prazeres et al. were estimated as K m = 23.6 μmoles FFA/ml, K i 1 = 4.6 × 10 −5 μmoles FFA/mg enzyme h, K i 2 = 0.0062 μmoles FFA/mg enzyme h and K 2 = 149.5 μmoles FFA/mg enzyme h.