Kinetic Study on Decolorization of the Dye Acid Orange Using the Fungus Phanerochate Chrysosporium

K.V Radha,Balu Balu

doi:10.5539/mas.v3n7p38

Abstract

A dye decolorizing fungal strain, Phanerochaete chrysosporium MTC 787 was used in batch experiments for decolorization of the dye, Acid orange10. Effects of initial substrate (dye) and fungal concentrations on the rate and extent of dye decolorization were investigated. The initial pH and oxidation–reduction potential (ORP) was kept at 4.5 and ?250 mV, respectively. The rate and extent of dye decolorization increased with increasing dye concentration up to 100 g l?1 and then decreased for larger dye concentrations. The rate of enzyme utilization and dye decolorization also increased linearly with increasing initial biomass concentration. A kinetic model describing the rate of enzyme utilization and substrate inhibition as function of the initial substrate and flow rate was developed. The kinetic constants were determined using the experimental data. The initial biomass should be above 3.2 x 105cell/ml to obtain high rates and decolorization percentage and to avoid substrate inhibition.

Highlights

A dye decolorizing fungal strain, Phanerochaete chrysosporium MTC 787 was used in batch experiments for decolorization of the dye, Acid orange10
Complete enzyme utilization took place when dye concentration was larger than 100 g l−1 (Fig 2a) due to substrate inhibition at high dye concentrations
The pH values dropped from an initial level of 4.5 to 3.0 at the end of 72 h when dye was less than 100 g l−1

Summary

Introduction

A dye decolorizing fungal strain, Phanerochaete chrysosporium MTC 787 was used in batch experiments for decolorization of the dye, Acid orange. The white-rot fungi best involved for treating dyes is P. chrysosporium and we have chosen the same for our reactor studies as the enzymes produced by the fungus which causes decolorization are highly non-specific and degrade a variety of dyes (Radha 2005). Many other parameters affect the capacity of microorganism to bind on the immobilizing agents (Wu 2006).The combined effects of two or more component of the microorganism depend on the nature of dye They compete for the binding sites and in combination of one or more dyes, competence is for the order of the dye addition and it includes the residence time in the reactor which may initiate the competence level (Aksu 2003).In order for the better utilization of the enzyme and the whole cells they are immobilized onto a solid support which leads to advantageous reactor operation system. 2009 solution for a large range of dye concentration and biomass concentrations and to determine the kinetic constants using the experimental data

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Conclusion