Abstract
p-Coumaric and 3- O-p-coumarylquinic acid seem to be important precursors of chlorogenic acid in the leaves of Cestrum poeppigii. 3- O-Cinnamylquinic acid, which has a very small metabolic activity, is of little importance in this respect. The kinetics of incorporation of radioactivity from t-cinnamic acid-3-[ 14C] into p-coumaric, 3- O-p-coumarylquinic, chlorogenic and 3- O-cinnamylquinic acid showed that the biosynthetic rates for these products decrease in the order shown. For p-coumaric acid, which has a markedly high metabolic activity, a turnover rate of 28 μg/hr and per gram fresh plant leaf, was calculated. Some trapping experiments with caffeic acid, and the acids mentioned above and using either t-cinnamic acid-3-[ 14C] or p-coumaric acid-2-[ 14C] as precursor, are discussed. A HPLC method for the rapid determination of phenolic acids in plant extracts, is described.
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