Abstract
Diacylglycerol (DAG) and monoacylglycerol (MAG) are two natural occurring minor components found in most edible fats and oils. These compounds have gained increasing market demand owing to their unique physicochemical properties. Enzymatic glycerolysis in solvent-free system might be a promising approach in producing DAG and MAG-enriched oil. Understanding on glycerolysis mechanism is therefore of great importance for process simulation and optimization. In this study, a commercial immobilized lipase (Lipozyme TL IM) was used to catalyze the glycerolysis reaction. The kinetics of enzymatic glycerolysis reaction between triacylglycerol (TAG) and glycerol (G) were modeled using rate equation with unsteady-state assumption. Ternary complex, ping-pong bi-bi and complex ping-pong bi-bi models were proposed and compared in this study. The reaction rate constants were determined using non-linear regression and sum of square errors (SSE) were minimized. Present work revealed satisfactory agreement between experimental data and the result generated by complex ping-pong bi-bi model as compared to other models. The proposed kinetic model would facilitate understanding on enzymatic glycerolysis for DAG and MAG production and design optimization of a pilot-scale reactor.
Highlights
The prevalence of overweight and obesity owing to the imbalance between energy intake and energy expenditure has triggered attention to the exploitation of an effective approach such as the development of structured-lipid, starch or protein-based fat replacer as well as food supplements and medication, to reverse the fasting growing obesity trend
Referring to Eqs 1–3, simple ternary model proposed in this study suggested that enzymatic glycerolysis reaction follows ordered-sequential bi-bi mechanism in which substrate TAG was initially bound to the enzyme (E) to form a binary complex (E.TAG)
The values of the rate constants and statistical evaluation of kinetic models with respect to experimental data are tabulated in Tables 1 and 2, respectively
Summary
The prevalence of overweight and obesity owing to the imbalance between energy intake and energy expenditure has triggered attention to the exploitation of an effective approach such as the development of structured-lipid, starch or protein-based fat replacer as well as food supplements and medication, to reverse the fasting growing obesity trend. Recent disclosure of diacylglycerol (DAG)-enriched oil has become a subject of growing interest worldwide. DAG in synergistic with monoacylglycerol (MAG) can act as excellent emulsifiers and stabilizers in food and pharmaceutical industry [4]. MAG alone has been widely employed in textile processing, production of plastics and formulation of oil for machinery on account of its excellent lubricant and plasticizing properties. The demand for both DAG and MAG has skyrocketed in recent years [5,6,7]
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