Abstract
Abstract Thermokinetic behaviors of α-amylase in the process of starch hydrolysis were studied under the conditions of different temperatures, different pH and metal ions by using thermokinetics theory and reduced extent method; the Michaelis constant (Km), apparent Michaelis constant ( K ′ m ), maximum velocity (Vmax) and apparent maximum velocity ( V ′ max ) of the α-amylase catalyzed reaction were obtained, Km–T and Km–pH equation were deduced. According to Km–T and Km–pH equation, the optimum temperature is 315.04 K and the optimum acidity is pH = 5.08. At the conditions of T = 313.15 K and pH = 5.02, the influence of metal ions (Ag+, Cu2+ and Zn2+) in α-amylase catalyzed reaction were studied; according to the data, it was discovered that the inhibitory type were versa-competitive inhibition and inhibitory consecution were Ag+ > Cu2+ > Zn2+ at the same concentration.
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