Kinetic study for the effect of new inhibitors on the activity of purified GPT from blood of cardiovascular patients

Abstract

In this study several biochemical parameters for cardiovascular patients have been measured CK-MB ,LDH ,GPT and GOT in addition to lipid profile , MDA ,Vitamin C and Vitamin E . New derivatives of ascorbic acid have been synthesized and tested to inhibit the purified GPT from the blood of cardiovascular patients Glutamate pyruvate transaminase (GPT) was purified from cardiovascular patients using ammonium sulphate salt for precipitation then dialysed using 0.1 M Tris-HCL buffer pH7.8 , then filtrated by gel filtration chromatography using Sephadex G-100 , followed by electrophoresis using poly acrylamide – bis acrylamide and sodium dodecyl sulphate (SDS). Derivatives of 3-(acetyl salicyloyl)-5,6 –O-isopropylidene-L-ascorbic acid (1) 2,3-di(acetyl salicyloyl)-5,6 –O-isopropylidene-L-ascorbic acid (2) and effect of 2,3,5,6 -tetra(acetyl salicyloyl)-L-ascorbic acid (3) which have been synthesized and characterized in this study , have been used in different concentrations to inhibit the purified enzyme activity using DMSO as a solvent .Results showed that these derivatives have inhibition effect on the enzyme activity with new value of kinetic parameters been detected using Linweaver-Burk plot and the type was un-competitive inhibition for the three derivatives been used in this study. Many biochemical parameters have been detected in this study including CK-MB, LDH, GPT and GOT as known marker for the disease in addition to lipid profile parameters Alb. UA, G6PDH, Vit.C and E as antioxidants as well as malondialdehyde (MDA). Results also showed significant differences in the levels of most parameters used in this study.