Abstract
The kinetics of sunflower oil transesterification with 1-butanol using a homogeneous lipase (Rhizomucor miehei) in an aqueous-organic biphasic system were studied in a stirred batch reactor set-up. An initial screening study was performed to optimize relevant process conditions (enzyme concentration, stirring speed, 1-butanol to oil ratio). A kinetic model was developed based on concentration time profiles. Important reaction parameters were the oil concentration in n-hexane, the enzyme concentration, the 1-butanol to oil molar ratio; the organic to an aqueous solvent volume ratio, the temperature, the pH setting using a phosphate buffer and the agitation rate. The experimental data were modelled using the Ping Pong Bi Bi mechanism with non-competitive inhibition by 1-butanol and a term for irreversible enzyme deactivation during reaction. Agreement between model and experiments was good (R2=0.99, average error 4.2%).
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