Abstract
1. 1. Pig erythrocyte hexokinase (ATP: d -hexose-6-phosphotransferase E.C.2.7.1.1) was isolated in a partially purified preparation by Sephadex G-100 gel filtration. 2. 2. In the mature pig (> 1 year of age), a high affinity component exhibits a maximum velocity at 0·2–0·4 mM glucose. This component seems to be inhibited by excessive glucose concentrations ( > 0·5 mM ). 3. 3. The young, adult pig (3–6 months of age) possesses a hexokinase which exhibits a maximum velocity at 1·0 μM glucose. 4. 4. 2,3-Diphosphoglycerate inhibits the enzyme at low concentrations of Mg 2+ (0·05 mM), but the inhibition is relieved as the concentration of Mg 2+ is increased beyond 1·0 mM. 5. 5. These findings indicate that the mature erythrocyte of the pig may possess unique biochemical properties that have not been observed in other mammalian red blood cells.
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