Abstract
Fast Ca 2+ release kinetics were measured in cardiac sarcoplasmic reticulum vesicles actively loaded with Ca 2+. Release was induced in solutions containing 1.2 mM free ATP and variable free [Ca 2+] and [Mg 2+]. Release rate constants ( k) were 10-fold higher at pCa 6 than at pCa 5 whereas Ryanodine binding was highest at pCa ≤5. These results suggest that channels respond differently when exposed to sudden [Ca 2+] changes than when exposed to Ca 2+ for longer periods. Vesicles with severalfold different luminal calcium contents exhibited double exponential release kinetics at pCa 6, suggesting that channels undergo time-dependent activity changes. Addition of Mg 2+ produced a marked inhibition of release kinetics at pCa 6 (K 0.5 = 63 μM) but not at pCa 5. Coexistence of calcium activation and inhibition sites with equally fast binding kinetics is proposed to explain this behavior. Thimerosal activated release kinetics at pCa 5 at all [Mg 2+] tested and increased at pCa 6 the K 0.5 for Mg 2+ inhibition, from 63 μM to 136 μM. We discuss the possible relevance of these results, which suggest release through RyR2 channels is subject to fast regulation by Ca 2+ and Mg 2+ followed by time-dependent regulation, to the physiological mechanisms of cardiac channel opening and closing.
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