Abstract
In the present study a kinetic-spectrophotometric method for thiocyanate determination is described. The suggested method for the determination of thiocyanate is based on its "Landolt effect" on the reaction of bromate with hydrobromic acid, which leads to the formation of only one halogen bromine. The reaction was monitored spectrophotometrically at the maximum wavelength of astrafloxine FF light absorption at 535 nm. The absorbance of reactants mixture decreased with an increase of the reaction time. The calibration curve for thiocyanate determination was obtained in the concentration range of 0.03-2.0 μg mLμ1 under the optimal conditions (pH 1.5; CBrO3 - = 7.6 × 10-4 mol L-1; Castrafloxine FF = 1 × 10-5 mol L-1). The limit of detection was 0.01 μg mLμ1. The method was successfully applied to the determination of thiocyanate in human saliva samples with satisfactory results.
Highlights
The toxicity of thiocyanates attracts the attention of specialists in various fields, such as medicine, ecology, food technology, etc., to the problem of developing new and improving existing methods for their determination.[1]
We describe the development of a new method for the determination of thiocyanate, based on its “Landolt effect” on the reaction of bromate with hydrobromic acid, which leads to the formation of bromine
Thiocyanate is a Landolt reagent in the reaction with bromate because the presence of thiocyanate in the solution causes the consumption of the produced Br2, and its reaction with thiocyanate is much faster than dye bromination
Summary
The toxicity of thiocyanates attracts the attention of specialists in various fields, such as medicine, ecology, food technology, etc., to the problem of developing new and improving existing methods for their determination.[1]. In biological fluids thiocyanates are formed as a result of detoxification of cyanides in the liver.[2] The most common source of inorganic cyanide in the human body is tobacco smoke. Since the content of thiocyanate in body fluids, especially saliva, increases with a constant source of cyanide, which is tobacco smoke, the concentration of thiocyanate in saliva, urine and serum is used as a biomarker to detect smokers.[3,4]. Various analytical methods have been proposed for the determination of thiocyanates. These include the potentiometry with ion-selective electrodes,[5] electrophoresis,[6] gas chromatography, and sequential-injection analysis or extraction-spectrophotometric methods.[7,8,9,10]
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