Kinetic spectrophotometric determination of Bi(III) based on its catalytic effect on the oxidation of phenylfluorone by hydrogen peroxide

Abstract

A new reaction was suggested and a new kinetic method was elaborated for determination of Bi(III) in solution, based on its catalytic effect on the oxidation of phenyl-fluorone (PF) by hydrogen peroxide in ammonia buffer. By application of spectrophotometric technique, a limit of quantification (LQ) of 128 ng cm-3 was reached, and the limit of detection (LD) of 37 ng cm-3 was obtained, where LQ was defined as the ratio signal: noise = 10:1 and LD was defined as signal 3:1 against the blank. The RSD value was found to be in the range 2.8-4.8 % for the investigated concentration range of Bi(III). The influence of some ions upon the reaction rate was tested. The method was confirmed by determining Bi(III) in a stomach ulcer drug ('Bicit HP', Hemofarm A.D.). The obtained results were compared to those obtained by AAS and good agreement of results was obtained.