Abstract
The kinetic performance of monolithic and fused-core capillary C18 columns was compared in isocratic-elution liquid chromatography. Heptanophenone was chosen as a test compound. The (u0, H) couple obtained in a column with permeability Kv0 was transferred into a plate number, dead time (N, t0) couple and the kinetic plots were achieved by using a Kinetic Method Plot Analyzer template. The u0,opt (0.98mm/s for the monolith and 1.05mm/s for the fused-core column) and Hmin (17.58μm for the monolith and 18.08μm for the fused-core column) values determined from the van Deemter curve were found to be almost identical, whereas the Nopt (the plate number for which the support achieves its best analysis time/pressure cost ratio) (150,000 for monolith and 41,000 for fused-core column), maximal plate number, Nmax (the maximum plate number that can ever be reached with a support) (550,000 for monolith and 120,000 for the fused-core column) and optimal analysis time, topt (the time needed to obtain Nopt plates) (18,583s for the monolith and 4,068s for the fused-core column) performance numbers determined from the kinetic plots were found to be significantly different.
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