Kinetic Parameters of Fed-Batch Production of Carotenoids bySporidiobolus salmonicolorUsing Low-Cost Agro-Industrial Substrates

Abstract

This work studied the optimization of carotenoids production by Sporidiobolus salmonicolor (CBS 2636) in a fed-batch bioreactor using coproducts (crude glycerol, corn steep liquor, rice parboiling water and yeast hydrolyzate). The maximum total carotenoids concentration, cell productivity and total were 4753 μg/L, 0.076 g/L·h and 48 μg/g, respectively, with a flow rate of 112 mL every 12 h, using a medium composed of 80 g/L crude glycerol, 80 g/L corn steep liquor and 20 g/L rice parboiling water at 25°C, pHinitial 4.0, agitation rate of 180 rpm, aeration rate of 1.5 vvm and 96 h of bioproduction. The specific maximum growth speed (μmax) was 0.052/h, obtaining the major carotenoid all-trans-γ-carotene (49%).