Kinetic Motilities of Cryopreserved Bull Spermatozoa: Owing to the Effect of &lt;i&gt;Eurycoma longifolia&lt;/i&gt; Jack Aqueous Extract

Falah Hasan Baiee,Salman Hammadi,Zaid Jeber,Muhammad Umar,Abd Wahid Haron,Tarig Aldelrouf Ahmedeltayeb,Rosnina Haji Yusoff,Mohamed Ariff Omar,Nurhusien Yimer,Wan-Nor Fitri

doi:10.3844/ajavsp.2017.77.84

Abstract

This study was carried out to improve the kinetic motilities of frozen-thawed bull semen diluted with tris-based egg yolk diluent that was supplemented with Eurycoma longifolia jack aqueous extract. A total of 24 ejaculates were obtained from six cross-bred bulls using an electro-ejaculator. The extract of Eurycoma longifolia jack was distributed into three low doses and three high doses; cryopreserved samples were evaluated into three different times to confirm the results of kinetic motilities through different times and between groups. Path velocity (VAP μm/s), progress velocity (VSL μm/s), track speed (VCL μm/s), lateral amplitude (ALH μm/s), Beat Frequency (BCF Hz), straightness (STR %), linearity (LIN %), were evaluated three different times using Computer-assisted sperm analysis. Results revealed that the percentage of VAP, ASL and VCL were higher (p<0.05) in the frozen-thawed semen group supplemented with 5 mg mL-1 Eurycoma longifolia jack extract (73.19±1.91, 58.34±2.06 and 117.91±2.68 in first evaluation, then 74.22±2.06, 57.45±1.72 and 118.92±2.55 in second evaluation and 72.95±2.27, 56.75±1.30 and 119.07±3.54 in third evaluation; respectively). In conclusion, Eurycoma longifolia Jack aqueous extract supplementation to the semen diluent at 5 mg mL-1 significantly improved sperm kinetic motilities of frozen-thawed bull semen.

Highlights

The energy and the nutrient materials which maintain mature spermatozoa are found in spermatozoon itself or in their environment
The outcomes of different doses of Eurycoma longifolia Jack (EL) extract that were supplemented into tris-base egg yolk diluent on frozenthawed kinetic motility parameters in bull spermatozoa for 1st, 2nd and 3rd evaluation are summarized in Table 2-4 and Fig. 3, respectively
The percentage of VAP, VSL and VCL were higher (p

Summary

Introduction

The energy and the nutrient materials which maintain mature spermatozoa are found in spermatozoon itself or in their environment. In semen that is collected in vitro using one of semen collection methods, spermatozoa utilize what they found in seminal plasma and semen diluent, but, in that case, the energy and nutrient materials are usually limited, leading to decrease the ability of sperm motility and increase the oxidative stress. The motility rate of frozen semen decreased owing to freezing shock (Peña et al, 2009; Yimer et al, 2015), decrease the pH in semen medium (Contri et al, 2013) and the oxidative stress (Ashrafi et al, 2013). The ability of frozen semen to incubation at body temperature for long period is sharply declined as compared to fresh or chilled semen (Ahmad et al, 2015)

Objectives

Methods

Results

Conclusion