Abstract
BackgroundShewanella baltica KB30 was isolated from seawater collected in Kandalaksha Bay, White Sea (Russia). This strain is known for its ability to grow on a pool of different substrates, including carbohydrates, carboxylic and amino acids, and lipids. However, no data are available on its metabolic efficiency in relation to the use of different carbon sources typologies. This work represents the first attempt to characterize S. baltica by its heterotrophic kinetic performance.ResultsGrowth and substrate consumption, during the biodegradation of sodium acetate, glucose, tween 80 and peptone, were analyzed through a respirometric method. To find the model best fitting the experimental data and to obtain the kinetic parameters, the equations of Monod, Moser, Contois and Tessier were applied. The kinetic behavior of S. baltica was fitted to Monod model for sodium acetate and tween 80, while it was adjusted to Contois model for glucose and peptone. In this regard, peptone was consumed faster than the other substrates, as indicated by the highest values of substrate degradation rate, which exceeded 60 mg O2 L−1 h−1.ConclusionsProteolytic metabolism was favored than lipidic and glucidic metabolism, which could contribute much more to mineralization and recycling of proteins than lipids and carbohydrates.
Highlights
Shewanella baltica KB30 was isolated from seawater collected in Kandalaksha Bay, White Sea (Russia)
Microorganism and culture conditions The strain of S. baltica KB30 used in this study was isolated in a previous work from seawater collected in Kandalaksha Bay (White Sea, Russia) [8], maintained at 4 °C in the microorganism culture collection of DEB (Department of Ecological and Biological Sciences, University of Tuscia) and sub-cultured on plate count agar (PCA, Difco, USA) slants when necessary
The KB30 specific metabolic competences had never been discussed in details and no further investigations, indicating which metabolic choices are favored by the strain, were done
Summary
Shewanella baltica KB30 was isolated from seawater collected in Kandalaksha Bay, White Sea (Russia). One of the basic tools in microbiology is the study of the relationships between the use of different substrates and the relative specific growth rate, giving information on the efficiency of substrate consumption in relation to different metabolic strategies. This kind of investigation, possibly supported by adequate kinetic modeling, is helpful to understand the microbial approach in its primary metabolism and to predict possible biodegradation of organic compounds in natural and engineered environments [17]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
