Abstract
Ethanol production via the batch fermentation of sugarcane juice using immobilized yeast has been studied. The influence of glucose concentration, ethanol concentration, and cell concentration (biomass) on the process rate throughout the period of fermentation has been investigated. Initial cell concentration was found to be 4.60 g/L saccharomyces cerevisiae. Biomass, ethanol and glucose concentrations were measured at different time interval during fermentation. The experimental data obtained were fitted using a variety of models for yeast growth. The logistic model gave the best fitting and was the basis for the development of the overall kinetic model. For ethanol formation, different model based on the logistic model for yeast growth were used to fit the experimental data and the leudeking – piret model was adopted because of its good fit. The leudeking – piret model was also adopted for substrate consumption. The estimated values of the kinetic parameters in the developed model were μm=0.04216hr-1, Xm = 6.2652g/L, α = 24.87149g/g.hr, Yx/s = 0.18292g/g and m = 0.008171g/g.hr. Therefore, a model based on the logistic equation of yeast growth, growth associated production of ethanol, and consumption of glucose for biomass and maintenance was found to accurately fit the production of ethanol from sugarcane.
Highlights
The ever increasing demand for ethanol and ethanol-based products over the years has necessitated an imminent requirement for high yielding production strains and search for alternative economically viable production processes
The regression analysis of the same data was analyzed using Microsoft excel data analysis and the root mean square value was estimated to be 0.8270. Both equations perfectly fitted the yeast growth but the logistic equation was the best because of the closeness of its value to unity when compared to the excel analysis
Since the logistic model gave the best fitting, it was adopted as the developed model for yeast growth
Summary
The ever increasing demand for ethanol and ethanol-based products over the years has necessitated an imminent requirement for high yielding production strains and search for alternative economically viable production processes. Ethanol is a very important component used in the pharmaceuticals It is used in some part of the world (Brazil) as a major component in the production of gasohol (alcohol and gasoline). In Nigeria, ethanol is used in the production of perfumes and vinegar. It is used as beverage in occasions like burial ceremonies, marriages and other traditional events [4,5,6]
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