Kinetic mechanisms of glycine requirement for N-methyl- d-aspartate channel activation

Abstract

Glycine potentiates N-methyl- d-aspartate (NMDA) receptor-mediated responses via its interaction with a strychnine-insensitive glycine recognition site. We have previously shown that the potent glycine receptor antagonist 7-chlorokynurenic acid (7C1-KYN) dose-dependently inhibits [ 3H]MK-801 binding to the PCP receptor and that this effect is reversed by glycine. [ 3H]MK-801 binding to the PCP receptor within the NMDA receptor-gated ion channel is a measure of channel activation. Association of PCP receptor ligands is biexponential with the fast component of binding serving as a marker of activated NMDA channels. In the present study we utilize 7C1-KYN as a probe of the kinetic mechanism of the glycine effect upon NMDA receptor functioning. In the presence of l-glutamate, incubation with 7Cl-KYN completely abolished the fast component of [ 3H]MK-801 association in 4 out of 5 experiments. In the fifth experiment where the fast component was detected, it accounted for less than half of that seen in the presence of l-glutamate alone. 7Cl-KYN-induced inhibition of the fast component of [ 3H]MK-801 association was reversed by the addition of glycine. Since the fast component represents ligand binding to the PCP receptor via the open NMDA channel, selective reduction of this component by 7Cl-KYN indicates that glycine receptor antagonists reduce the probability of channel opening, and also that the selective reduction in the component of [ 3H]MK-801 binding that manifest fast kinetics can serve as a marker for glycine antagonists.