Abstract
Apocynin has been reported to require dimerization by myeloperoxidase (MPO) to inhibit leukocyte NADPH oxidase. (–)-Epicatechin, a dietary flavan-3-ol, has been identified as a ‘prodrug’ of apocynin-like metabolites that inhibit endothelial NADPH oxidase activity and elevate the cellular level of nitric oxide. Since (–)-epicatechin has tentatively been identified as substrate of MPO, we studied the one-electron oxidation of (–)-epicatechin by MPO. By using multi-mixing stopped-flow technique, we demonstrate that (–)-epicatechin is one of the most efficient electron donors for heme peroxidases investigated so far. Second order rate constants for the (–)-epicatechin-mediated conversion of MPO-compound I to compound II and compound II to resting enzyme were estimated to be 1.9 × 10 7 and 4.5 × 10 6 M −1 s −1, respectively (pH 7, 25 °C). The data indicate that (–)-epicatechin is capable of undergoing fast MPO-mediated one-electron oxidation.
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