Abstract
A kinetic method for the determination of morphine in urine is presented. It is based on the decomposition of the coloured compound formed by the reaction between hydrogen peroxide, cobalt(II) and morphine, in alkaline media. The decomposition rate of the coloured reaction product was followed photometrically. Morphine was determined in concentrations ranging from 1.5 to 13.5μg/ml, and the relative standard deviation was not higher than 8%. The determination was performed by the differential tangent method. The effect of related substances from the opium alkaloid group was also examined. The method determines the free morphine present. For total morphine the glucuronides have to be hydrolysed by acid digestion first.
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