Abstract
1. 1. Previous studies have shown that in Mytilus edulis NADP-isocitrate dehydrogenase exhibits a rapid-equilibrium random-ordered reaction mechanism. The relative reaction rate v/ V max can be calculated from the initial rate equation including product inhibition by NADPH. 2. 2. The concentrations of d-isocitrate and the total (NADP + NADPH) pool have been determined in the digestive gland and mantle tissue of M. edulis. The levels of α-ketoglutarate are very low so that inhibition will be negligible compared with NADPH. 3. 3. At physiological concentrations of d-isocitrate, between values of K m and 10 K m , the reaction rate is controlled by the NADP/NADPH ratio. Small changes in the concentration of d-isocitrate do not affect the reaction rate. 4. 4. At saturating levels of d-isocitrate, any utilization of NADPH for biosynthesis will be followed by a rise in the redox ratio and an increase in the production of more NADPH.
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