Kinetic behavior of Candida guilliermondii yeast during xylitol production from highly concentrated hydrolysate

Abstract

Rice straw hemicellulosic hydrolysate containing a high xylose concentration was used as fermentation medium to evaluate the kinetic behavior of Candida guilliermondii yeast (FTI 20037) during the bioconversion of xylose into xylitol. Assays were conducted first with detoxified and non-detoxified (raw) hydrolysates and semi-synthetic medium in agitated flasks, and second with detoxified hydrolysate in a stirred-tank bioreactor at a given oxygen transfer rate. The results for the agitated flasks showed that in detoxified hydrolysate the xylose-to-xylitol bioconversion by the yeast was as effective as in synthetic medium and 47% higher than in raw hydrolysate. In the stirred-tank bioreactor, the kinetic behavior of the yeast in detoxified hydrolysate was slower, resulting in smaller values of fermentative parameters, probably due to unsuitability of the oxygen transfer rate employed ( K L a=22 h −1).