Abstract
Docosahexaenoic acid (DHA) is one of the most important long-chain polyunsaturated fatty acids (LC-PUFAs), with numerous health benefits. Crypthecodinium cohnii, a marine heterotrophic dinoflagellate, is successfully used for the industrial production of DHA because it can accumulate DHA at high concentrations within the cells. Glycerol is an interesting renewable substrate for DHA production since it is a by-product of biodiesel production and other industries, and is globally generated in large quantities. The DHA production potential from glycerol, ethanol and glucose is compared by combining fermentation experiments with the pathway-scale kinetic modeling and constraint-based stoichiometric modeling of C. cohnii metabolism. Glycerol has the slowest biomass growth rate among the tested substrates. This is partially compensated by the highest PUFAs fraction, where DHA is dominant. Mathematical modeling reveals that glycerol has the best experimentally observed carbon transformation rate into biomass, reaching the closest values to the theoretical upper limit. In addition to our observations, the published experimental evidence indicates that crude glycerol is readily consumed by C. cohnii, making glycerol an attractive substrate for DHA production.
Highlights
Introduction published maps and institutional affilKnowledge-based bioeconomy implies the conversion of cheap renewable resources into biotechnological products with added value.Docosahexaenoic acid (DHA) is one of the most important long-chain polyunsaturated fatty acids (LC-PUFAs), with numerous health benefits such as reducing the risk of cardiovascular diseases, cancer, and rheumatoid arthritis; alleviating depression symptoms and post-natal depression; and contributing to immune-modulatory effects [1]
Three experimental parameter sets have been developed for the kinetic model to account for different substrate uptakes: glucose, glycerol and ethanol
The same parameter set of kinetic models could be used for glucose and glycerol experiments
Summary
Introduction published maps and institutional affilKnowledge-based bioeconomy implies the conversion of cheap renewable resources into biotechnological products with added value.Docosahexaenoic acid (DHA) is one of the most important long-chain polyunsaturated fatty acids (LC-PUFAs), with numerous health benefits such as reducing the risk of cardiovascular diseases, cancer, and rheumatoid arthritis; alleviating depression symptoms and post-natal depression; and contributing to immune-modulatory effects [1]. Knowledge-based bioeconomy implies the conversion of cheap renewable resources into biotechnological products with added value. Docosahexaenoic acid (DHA) is one of the most important long-chain polyunsaturated fatty acids (LC-PUFAs), with numerous health benefits such as reducing the risk of cardiovascular diseases, cancer, and rheumatoid arthritis; alleviating depression symptoms and post-natal depression; and contributing to immune-modulatory effects [1]. DHA has an important role in the healthy development of the fetal brain and retina, and is commonly used in infant-related food products. The global EPA/DHA market was estimated at USD 2.49 billion in 2019, with a projected annual growth rate of 7% until. Cold-water marine fish oil is a source of 96% of DHA, but it is not able to meet the increasing demand of DHA for human consumption [3] due to the depletion of wild fish stocks and pollution of the marine environment
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